![]() ![]() neoformans with special focus on its mode of action. The aim of the present study was to evaluate the inhibitory effect of aureobasidin A on C. ![]() albicans biofilm in FESEM analysis suggests an anti-biofilm effect of AbA.Conclusions:The variability in the growth characteristics of Candida biofilm cultures affects susceptibility to AbA, with higher susceptibility noted in biofilm cultures exhibiting filamentous form and high biomass/metabolic activity.Ĭryptococcosis is an opportunistic fungal infection caused mainly by Cryptococcus neoformans. The observation of filament detachment and the almost complete removal of biofilm from AbA-treated C. Candida isolates with filamentous growth, higher biomass and metabolic activity showed lower AbA MIC50(at least fourfold), compared to those exhibiting yeast morphology, and lower biomass and metabolic activity. The biofilm producers were divided into two populations (low and high), based on the ability in producing biomass and their metabolic activity. ![]() ![]() Two morphological variants of biofilms were identified in our isolates, with 48.6% of the isolates showing mainly yeast and pseudohyphae-like structures, while the remaining ones were predominantly filamentous forms. Candida parapsilosis was the largest producer, followed by Candida tropicalis and C. The anti-biofilm effect of AbA on Candida albicans was examined using field emission scanning electron microscope (FESEM) analysis.Results:A total of 35 (29.7%) of 118 Candida isolates were regarded as biofilm producers in this study. The determination of AbA planktonic and biofilm MICs was performed based on a micro-broth dilution method. Aureobasidin A (AbA), a cyclic depsipeptide targeting fungal sphingolipid biosynthesis, has been shown to be effective against several Candida species.Aims:The aim of this study was to investigate Candida biofilm growth morphology, its biomass, metabolic activity, and to determine the effects of AbA on the biofilm growth.Methods:The biofilm forming ability of several clinical isolates of different Candida species from our culture collection was determined using established methods (crystal violet and XTT assays). Background:Biofilm is known to contribute to the antifungal resistance of Candida yeasts. ![]()
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